PCR Supplies
Genscript Corporation M-MuLV Reverse Transcriptase
GenScript M-MuLV Reverse Transcriptase (M-MLV) is derived from a cloned region of the pol gene of MMLV and isolated from an E coli strain overexpressing this construct To increase cDNA yields and get a higher percentage of longer transcripts the M-MLV Reverse Transcriptase has been modified with reduced RNase H activity and expressed free of exogenous RNases and other nucleases The enzyme can synthesize a complementary cDNA strand initiating from a primer using RNA as template (cDNA synthesis) making it ideal for a wide range of applications
Meridian Life Science MMLV-RT, 20ML
MMLV-RT is a high-quality reverse transcriptase that synthesizes a complementary DNA (cDNA) strand from mRNA or total RNA. With higher thermostability and reduced RNase H activity, it can be used for synthesis with long messenger RNA templates with high sensitivity and efficiency and is ideal for detecting low-level target genes.
Meridian Life Science Lyo-Compatible MMLV-RT, 80
Lyo-Compatible MMLV-RT is a high-concentration, Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RT) with reduced RNase H activity, which exhibits high sensitivity and efficiency for cDNA synthesis and is suitable for incorporation into wet or lyophilized multiplex RT-PCR assays, allowing high sensitivity detection of low copy number RNA targets.
Worthington Biochemical Corporation REVERSE TRANSCRIPTASE 200UN
Chromatographically purified dimeric form with M.W. of 66 kDa and 51 kDa. A solution in 10 mM potassium phosphate, pH 7.4, 1 mM DTT and 20% glycerol. 9068-38-6
Agilent Technologies AffinityScript Multiple Temperature Reverse Transcriptase, 200 reactions per kit
Our AffinityScript Multiple Temperature Reverse Transcriptase is engineered to be highly thermostable, allowing you to reverse transcribe at your preferred reaction temperature.
INTACT GENOMICS INC igScript™ Reverse Transcriptase 50,000 Units (200 u/µl)
igScript™ Reverse Transcriptase (igScript RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. igScript RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.Applications: First strand cDNA synthesis for PCR or RT-PCR Gene expression data validation by using RT-PCRBenefits: Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. Active at temperatures up to 55°C. Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA.Product Includes: igScript™ Reverse Transcriptase 10x igScript™ RT Reaction Buffer
Agilent Technologies AFFINITYSCRIPT MULT TEMP TRANS
Our AffinityScript Multiple Temperature Reverse Transcriptase is engineered to be highly thermostable, allowing you to reverse transcribe at your preferred reaction temperature.
Growcells PCR GRADE H2O PK100
Sterile PCR grade nuclease free water. Prepared with 18.2 megOhm, 0.1 micron sterile filtered water and autoclaved.
New England Biolabs, Inc. Bst 3.0 DNA Polymerase – 1600 units
Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5' to 3' DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5' to 3' and 3' to 5' exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
Abcam Acyclovir, viral DNA polymerase inhibitor, 100MG
MW 225.21 g/mol, Purity >99%. Guanosine derivative, competitively inhibits viral DNA polymerase. High specificity for herpes simplex 1 and 2 (EC₅₀ values are 0.04 and 0.44 μM, respectively) and varicella-zoster viruses (IC₅₀ = 0.22 μM).
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. phi29 DNA Polymerase – 1250 units
phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29. This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3' to 5' proofreading exonuclease activity.